Application
Western blot analysis of MAG Antibody in A375 cell line lysates (35ug/lane)Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Myelin-associated glycoprotein, Siglec-4a, MAG, GMA |
| Product description | MAG is a type I membrane protein and member of the immunoglobulin uperfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions.1) Stalder,A.K.,et.al.,J. Neuropathol. Exp. Neurol. 68 (2), 148-158 (2009) |
| Immunogen | This MAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-466 amino acids from the Central region of human MAG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAG |
|---|---|
| Alternative Names | Myelin-associated glycoprotein Siglec-4a MAG GMA |
| Molecular Weight(MW) | 69 kDa |
Application
Western blot analysis of MAG Antibody in A375 cell line lysates (35ug/lane)
Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM284016