Application
Immunohistochemistry of MAP1LC3 in rat brain tissue with MAP1LC3 antibody at 5 μg/ml.Application
Western blot analysis of MAP1LC3 in human brain tissue lysate with MAP1LC3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | MAP1LC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3 is a subfamily of three related proteins belonging to the MAP1 LC3 family and it includes MAP1LC3A, MAP1LC3B, and MAP1LC3C (2). MAP1LC3 is the mammalian homolog of yeast ATG8 and is essential for autophagy and associated with the autophagosome membranes after processing (3). The three isoforms exhibit distinct expression patterns and both MAP1LC3A and MAP1LC3B but not MAP1LC3B, are post-translationally modified, suggesting the three isoforms may have different physiological functions (4).1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81. |
| Immunogen | MAP1LC3 antibody was raised against a 12 amino acid peptide near the center of human MAP1LC3A. The immunogen is located within amino acids 20 - 70 of MAP1LC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3A |
|---|---|
| Alternative Names | MAP1LC3 Antibody: LC3 LC3A ATG8E MAP1ALC3 MAP1BLC3Autophagy-related protein LC3 A |
| Molecular Weight(MW) | Predicted: 13 kDa Observed: 18 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | MAP1LC3 antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3 are known to exist. MAP1LC3 antibody is predicted to detect MAP1LC3A, MAP1LC3B, and MAP1LC3C. |
Application
Immunohistochemistry of MAP1LC3 in rat brain tissue with MAP1LC3 antibody at 5 μg/ml.
Application
Western blot analysis of MAP1LC3 in human brain tissue lysate with MAP1LC3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
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| Application Notes | MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MAP1LC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MAP1LC3 antibody is supplied in PBS containing 0.02% sodium azide. |
