Application
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 μg/mL.Application
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 μg/mL.Application
Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.| Product Name | MAP1LC3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81. |
| Immunogen | MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A. The immunogen is located within the first 50 amino acids of MAP1LC3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3A |
|---|---|
| Alternative Names | MAP1LC3A Antibody: LC3 LC3A ATG8E MAP1ALC3 MAP1BLC3Autophagy-related protein LC3 A |
| Molecular Weight(MW) | Predicted: 14 kDa Observed: 17 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C. |
Application
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 μg/mL.
Application
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 μg/mL.
Application
Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MAP1LC3A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MAP1LC3A antibody is supplied in PBS containing 0.02% sodium azide. |
