Application
Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μg/mL.Application
Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μg/mL.Application
Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μg/ml.| Product Name | MAP1LC3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81. |
| Immunogen | MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3B |
|---|---|
| Alternative Names | MAP1LC3B Antibody: LC3B ATG8F MAP1LC3B-a MAP1A/1BLC3 MAP1ALC3 Autophagy-related protein LC3 B |
| Molecular Weight(MW) | Predicted: 14 kDa Observed: 14 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C |
Application
Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μg/mL.
Application
Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μg/mL.
Application
Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MAP1LC3B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide. |
