ICC/IF
Immunofluorescence analysis of A549 cells using LC3B antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-LC3B antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP1LC3B |
|---|---|
| Gene Synonyms | 1010001C15Rik 1010001H21Rik 4922501H04Rik Atg8 ATG8E ATG8F ATG8G LC3 LC3-I LC3-II LC3A LC3B MAP1A/1BLC3 MAP1A/MAP1B MAP1ALC3 MAP1BLC3 Map1lc3 MAP1LC3B-a Mpl3 wu:fb60g11 zbs559 zgc:56434 zgc:77094 |
| Gene Full Name | microtubule associated protein 1 light chain 3 beta |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| Alternative Names | autophagy-related protein LC3 A autophagy-related protein LC3 B autophagy-related ubiquitin-like modifier LC3 A autophagy-related ubiquitin-like modifier LC3 B LC3 LC3-like protein 1 lc3a lc3b lc3b3 MAP1 light chain 3-like protein 1 MAP1 light chain 3-like protein 2 MAP1A/1B MAP1A/1B LC3 MAP1A/1B light chain 3 A MAP1A/1B light chain 3 B MAP1A/MAP1B LC3 A MAP1A/MAP1B LC3 B MAP1A/MAP1B LC3 B-like MAP1A/MAP1B light chain 3 A MAP1A/MAP1B light chain 3 B MAP1A/MAP1B light chain 3 B-like MAP1A1B-LC3 microtubule-associated protein 1-light chain 3A microtubule-associated proteins 1A/1B light chain 3 microtubule-associated proteins 1A/1B light chain 3 beta 2 microtubule-associated proteins 1A/1B light chain 3A microtubule-associated proteins 1A/1B light chain 3B Microtubule-associated proteins 1A/1B light chain 3B-like protein |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule |
| SwissProt ID | Q9GZQ8 |
|---|
ICC/IF
Immunofluorescence analysis of A549 cells using LC3B antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
