Application
Western blot analysis of lysed extracts from HUVEC cells using MAP2K4 Antibody.| Product Name | MAP2K4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).1) Davis, R. J. (1994) Trends Biochem. Sci. 19, 470-473. Sanchez, I. et al. (1994) Nature 372, 794-798. Yan, M. et al. (1994) Nature 372, 798-800. Kyriakis, J. M. et al. (1994) Nature 369, 156-160. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578. |
| Immunogen | MAP2K4 antibody was raised against a peptide sequence around aa.352~356 (E-S-K-R-P) derived from Human MAP2K4. . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K4 |
|---|---|
| Alternative Names | JNKK MEK4 MKK4 SEK1 SKK1 JNKK1 SERK1 MAPKK4 PRKMK4 SAPKK1 SAPKK-1 |
| Molecular Weight(MW) | 44 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total MAP2K4 protein. |
| Entrez Gene | 6416 |
|---|---|
| Protein Accession | NP_003001.1 |
Application
Western blot analysis of lysed extracts from HUVEC cells using MAP2K4 Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
