Application
Western blot analysis of MAP2K4 using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | MEK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.1) Robinson,V.L.,Mol. Cancer Res.(3), 501-508 (2008) |
| Immunogen | This MEK4(MAP2K4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from human MEK4(MAP2K4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K4 |
|---|---|
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 4 MAP kinase kinase 4 MAPKK 4 JNK-activating kinase 1 MAPK/ERK kinase 4 MEK 4 SAPK/ERK kinase 1 SEK1 Stress-activated protein kinase kinase 1 SAPK kinase 1 SAPKK-1 SAPKK1 c-Jun N-terminal |
| Molecular Weight(MW) | 44 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Western blot analysis of MAP2K4 using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
