Application
Western blot analysis of MAPK12 using rabbit polyclonal hMAPK12-P354 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.Application
Western blot analysis of p38 gamma (MAPK12) Antibody in mouse lung tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | MAPK12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.1) Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003). |
| Immunogen | This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MAPK12 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 12 MAP kinase 12 MAPK 12 Extracellular signal-regulated kinase 6 ERK-6 Mitogen-activated protein kinase p38 gamma MAP kinase p38 gamma Stress-activated protein kinase 3 MAPK12 ERK6 SAPK3 |
| Molecular Weight(MW) | 42 kDa |
Application
Western blot analysis of MAPK12 using rabbit polyclonal hMAPK12-P354 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.
Application
Western blot analysis of p38 gamma (MAPK12) Antibody in mouse lung tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
