Application
HEK293T cells transfected with either MAPK12 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK12 antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12.Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MAPK12mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-MAPK12 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | p38 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10E1] to p38 gammaResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. |
| Immunogen | Protein expressed in 293T cell transfected with human p38 gamma expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E2 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAPK12 P38GAMMA ERK3 ERK6 PRKM12 SAPK3 SAPK-3 SAPK 3 |
|---|---|
| Target | p38 gamma |
| Tissue Specificity | is specific for human p38 gamma. |
| Cellular Localization | Mitochondrial when associated with SH3BP5. |
Application
HEK293T cells transfected with either MAPK12 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK12 antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12.
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MAPK12mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-MAPK12 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
