Application
Immunohistochemistry staining of MAPK3 in prostate tissue using MAPK3 Antibody.| Product Name | MAPK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3 |
| Product description | ERK1, a MAPK-type protein kinase, is a member of the Ras-MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins, and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK1 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK1 causing dissociation of the MEK/ERK complex. A portion of the activated ERK1 pool forms dimers and translocates to the nucleus to activate nuclear proteins such as ELK-1 and MSK1. Cytosolic ERK 1 acts on a different set of substrates, including MNK1/2 and MAPKAP-K1. |
| Immunogen | MAPK3 antibody was raised against a peptide located near the internal domain of MAPK3 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| MAPK3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | GibbonGorillaHumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK3 |
|---|---|
| Alternative Names | MAPK3 ERK-1 ERT2 HUMKER1A HS44KDAP MAP kinase 1 MAP kinase isoform p44 MAPK 1 MAPK 3 p44 p44MAPK p44-MAPK p44ERK1 Insulin-stimulated MAP2 kinase MAP kinase 3 p44-ERK1 ERK1 PRKM3 |
| Tissue Specificity | BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (70%). |
Application
Immunohistochemistry staining of MAPK3 in prostate tissue using MAPK3 Antibody.| Application Notes | MAPK3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MAPK3 antibody should be stored long term (months) at -80 ˚C and short term (days) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
