Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).| Product Name | p44 / 42 MAP Kinase (phospho Tyr204) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKC2, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1 |
| Product description | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1.1) TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321Hironobu Ihn et al. (2000) Immunology 165: 2149-2155 |
| Immunogen | p44/42 MAP Kinase (Phospho-Tyr204) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y (p) -V-A) derived from Human p44/42 MAP Kinase. |
| Modification | p-Tyr204 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK3 |
|---|---|
| Alternative Names | ERK1 ERT2 ERK-1 PRKM3 P44ERK1 P44MAPK HS44KDAP HUMKER1A p44-ERK1 p44-MAPK PKC2 ERK Extracellular signal- regulated kinase 1 Insulin-stimulated MAP2 kinase MAP kinase 1 |
| Molecular Weight(MW) | 42, 44 kDa |
| Tissue Specificity | This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204. |
| Entrez Gene | 5595 |
|---|---|
| Protein Accession | NP_001035145.1 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
