Application
Western blot analysis of MAPK3 using rabbit polyclonal MAPK3 Antibody (Y204) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2).| Product Name | MAPK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3 |
| Product description | MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.1) Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004). |
| Immunogen | This MAPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from human MAPK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK3 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 3 MAP kinase 3 MAPK 3 ERT2 Extracellular signal-regulated kinase 1 ERK-1 Insulin-stimulated MAP2 kinase MAP kinase isoform p44 p44-MAPK Microtubule-associated protein 2 kinase p44-ERK1 MAPK3 ERK1 PRKM3 |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Drosophila |
Application
Western blot analysis of MAPK3 using rabbit polyclonal MAPK3 Antibody (Y204) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
