Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | MARCH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.1) Cao, Z., (2008) Cell. Signal. 20 (1), 73-82. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MARCH2 antibody can be used for detection of 40239 by ELISA at 1:312500. MARCH2 antibody can be used for detection of 40239 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH2 |
|---|---|
| Alternative Names | MARCH2 HSPC240 MARCH-II RNF172 |
| Molecular Weight(MW) | 27 kDa |
| Entrez Gene | 51257 |
|---|---|
| Protein Accession | NP_001005415 |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MARCH2 antibody can be used for detection of 40239 by ELISA at 1:312500. MARCH2 antibody can be used for detection of 40239 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MARCH2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
