Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).| Product Name | MARCH9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM].1) Nice, T.J., et al. J. Immunol. 185(9):5369-5376(2010) |
| Immunogen | This MARCH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human MARCH9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH2 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase MARCH9 632- Membrane-associated RING finger protein 9 Membrane-associated RING-CH protein IX MARCH-IX RING finger protein 179 MARCH9 RNF179 |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
