Application
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).| Product Name | MARCKS (phospho Ser162) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.1) Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102 (35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280 (3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359 (2): 151-159 |
| Immunogen | MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS. |
| Modification | p-Ser162 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IF |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCKS |
|---|---|
| Alternative Names | MACS 80K-L PKCSL PRKCSL TSFP1 MACS MARCS Protein kinase C substrate |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162. |
| Entrez Gene | 4082 |
|---|---|
| Protein Accession | NP_002347.5 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
