Application
Western blot analysis of hMARCKS-K254 in Hela cell line lysates (35ug/lane)| Product Name | MARCKS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.1) Rauch, M.E., et al., J. Biol. Chem. 277(16):14068-14076 (2002). |
| Immunogen | This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MARCKS |
|---|---|
| Alternative Names | Myristoylated alanine-rich C-kinase substrate MARCKS Protein kinase C substrate 80 kDa protein light chain 80K-L protein PKCSL MARCKS MACS PRKCSL |
| Molecular Weight(MW) | 32 kDa |
Application
Western blot analysis of hMARCKS-K254 in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
