Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170).Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using MARCKS (phospho-Ser170).| Product Name | MARCKS (phospho Ser170) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.1) Ramsden, J. J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. Hartwig, J. H. et al. (1992) Nature 356, 618-622. Thelen, M. et al. (1991) Nature 351, 320-322. |
| Immunogen | MARCKS (phospho-Ser170) antibody was raised against a peptide sequence around phosphorylation site of Serine 170 (G-F-S (p) -F-K) derived from Human MARCKS. |
| Modification | p-Ser170 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCKS |
|---|---|
| Alternative Names | MACS 80K-L PKCSL PRKCSL HSP27 HSP28 Protein kinase C substrate 80 kDa protein light chain |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170. |
| Entrez Gene | 4082 |
|---|---|
| Protein Accession | NP_002347.5 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170).
Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using MARCKS (phospho-Ser170).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
