Application
Flow cytometric analysis of ATDC5 cells using MCCC2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).| Product Name | MCCC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCCC2 is the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA.1) Uematsu,M., et.al., J. Hum. Genet. 52 (12), 1040-1043 (2007) |
| Immunogen | This MCCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-189 amino acids from the Central region of human MCCC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCCC2 |
|---|---|
| Alternative Names | Methylcrotonoyl-CoA carboxylase beta chain mitochondrial MCCase subunit beta 3-methylcrotonyl-CoA carboxylase 2 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta MCCC2 MCCB |
| Molecular Weight(MW) | 61 kDa |
Application
Flow cytometric analysis of ATDC5 cells using MCCC2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
