Application
Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/mL.Application
Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 20 μg/mL.Application
Western blot analysis of Mcl-1in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Mcl-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.1) Edwards SW, Derouet M, Howse M, et al. Regulation of neutrophil apoptosis by Mcl-1. Biochem Soc Trans. 2004; 32:489-92. |
| Immunogen | Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Mcl-1. The immunogen is located within amino acids 20 - 70 of Mcl-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1S by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCL1 |
|---|---|
| Alternative Names | Mcl-1 Antibody: TM EAT MCL1L MCL1S Mcl-1 BCL2L3 MCL1-ES bcl2-L-3 mcl1/EAT Induced myeloid leukemia cell differentiation protein Mcl-1 Bcl-2-like protein 3 Bcl2-L-3 |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
| Tissue Specificity | Detects isoforms Mcl-1L and Mcl-1S |
Application
Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/mL.
Application
Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 20 μg/mL.
Application
Western blot analysis of Mcl-1in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1S by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Mcl-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
