Application
Western blot analysis using MCL - 1 monoclonal antibody against BCBL - 1 cell lysate.Application
Immunohistochemical analysis of paraffin - embedded human lymphnode showing cytoplasmic localization using MCL - 1 antibody with DAB staining.| Product Name | MCL1 Antibody [8C6D4B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.1) Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269. |
| Immunogen | Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:200 - 1:1,000 IHC(F):Dilution 1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCL1 |
|---|---|
| Alternative Names | TM EAT MCL1L MCL1S Mcl-1 BCL2L3 MCL1-ES bcl2-L-3 mcl1/EAT |
| Entrez Gene | 4170 |
|---|---|
| Protein Accession | NP_001184249.1 |
Application
Western blot analysis using MCL - 1 monoclonal antibody against BCBL - 1 cell lysate.
Application
Immunohistochemical analysis of paraffin - embedded human lymphnode showing cytoplasmic localization using MCL - 1 antibody with DAB staining.| Application Notes | Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:200 - 1:1,000 IHC(F):Dilution 1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
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| Form | Liquid |
|---|---|
| Storage Instructions | MCL-1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
