Application
Immunohistochemistry of MD-2 in human spleen tissue with MD-2 antibody at 2.5 μg/ml.Application
Immunohistochemical staining of rat spleen cells using MD-2 antibody at 2 μg/mL.Application
Immunofluorescence of MD-2 in Rat Spleen tissue with MD-2 antibody at 10 μg/mL.Application
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 μg/mL.| Product Name | MD-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.1) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. |
| Immunogen | MD-2 antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MD-2 antibody can be used for detection of MD-2 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LY96 |
|---|---|
| Alternative Names | MD-2 Antibody: MD2 MD-2 ly-96 ESOP-1 ESOP1 MD2 Lymphocyte antigen 96 Ly-96 |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of MD-2 in human spleen tissue with MD-2 antibody at 2.5 μg/ml.
Application
Immunohistochemical staining of rat spleen cells using MD-2 antibody at 2 μg/mL.
Application
Immunofluorescence of MD-2 in Rat Spleen tissue with MD-2 antibody at 10 μg/mL.
Application
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | MD-2 antibody can be used for detection of MD-2 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MD-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MD-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
