Application
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.Application
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.Application
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.| Product Name | MD-2 Antibody [9F1B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.1) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. |
| Immunogen | Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIFIHC-PWB |
| MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LY96 |
|---|---|
| Alternative Names | MD-2 Antibody [9F1B1] : MD2 MD-2 ly-96 ESOP-1 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.
Application
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 95-108 - MD-2 Recombinant Protein |
|---|---|
| Application Notes | MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MD-2 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
