| Product Name | MDC Antibody [1.3_1C6-1D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16. |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human MDC as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MDC when used with our biotinylated antigen affinity purified anti-human MDC (XP-5229Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 0.25-0.50 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL22 |
|---|---|
| Alternative Names | ABCD 1 CC chemokine STCP 1 CCL 22 MGC34554 SCYA22 Small inducible cytokine subfamily A Cys Cys member 22 STCP 1 Stimulated T cell chemotactic protein 1 MDC A-152E5.1 C-C motif chemokine 22 CC chemokine STCP-1MDC ABCD-1 STCP-1 DC/B-CK |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MDC when used with our biotinylated antigen affinity purified anti-human MDC (XP-5229Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 0.25-0.50 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MDC lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
