Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on Human MCF-7 at 1:1000 (Lanes 1-4: 10 ug MCF7 cell lysate).| Product Name | MDM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Gilkes, D.M., (2008) Mol. Cell. Biol. 28 (6), 1999-2010. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDM4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MDM4 antibody can be used for detection of MDM4 by ELISA at 1:12500. MDM4 antibody can be used for detection of MDM4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM4 |
|---|---|
| Alternative Names | MDM4 DKFZp781B1423 MDMX MGC132766 MRP1 HDMX |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 1:1000 (Lanes 1-4: 10 ug MCF7 cell lysate).| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | MDM4 antibody can be used for detection of MDM4 by ELISA at 1:12500. MDM4 antibody can be used for detection of MDM4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MDM4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
