Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Product Name | MDM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene.1) Xu, N., et al. Biochem. Biophys. Res. Commun. 401(3):417-421(2010) |
| Immunogen | This MDM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human MDM4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM4 |
|---|---|
| Alternative Names | Protein Mdm4 Double minute 4 protein Mdm2-like p53-binding protein Protein Mdmx p53-binding protein Mdm4 MDM4 MDMX |
| Molecular Weight(MW) | 55 kDa |
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
