Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2 (Ab-217/221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1/MEK2(Ab-217/221).Application
Western blot analysis of lysed extracts from 293 cells using MEK1/MEK2 (Ab-217/221).| Product Name | MEK1 / MEK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Shen R, et al. (2002) Mol Cell Biol; 22 (10): 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4): 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1): 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11): 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5): 2304-2310 |
| Immunogen | MEK1/MEK2 (Ab-217/221) antibody was raised against a peptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 MAP2K2 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 GLUR2 ERK activator kinase 1/ERK activator kinase2 MAP kinase kinase 1/MAP kinase kinase 2 MAP2K1/MAP2K1 MAPK/ERK kinase 1/MAPK/ERK kinase 2 MAPKK 1/MAPKK 1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MEK1/MEK2 protein. |
| Entrez Gene | 5604 / 5605 |
|---|---|
| Protein Accession | NP_002746.1 / NP_109587.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2 (Ab-217/221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1/MEK2(Ab-217/221).
Application
Western blot analysis of lysed extracts from 293 cells using MEK1/MEK2 (Ab-217/221).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
