Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2 (Phospho-Ser217/Ser221).Application
Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/MEK2 (Phospho-Ser217/Ser221) Antibody#11205WB
Tan IIA increases the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as detected by immunoblotting.IHC
Tan IIA increased the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as assessed by immunohistochemical analysis.WB
(E) Tan IIA increased the expression of p-MEK| Product Name | MEK1 / MEK2 (phospho Ser217 / Ser221) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Shen R, et al. (2002) Mol Cell Biol; 22 (10): 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4): 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1): 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11): 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5): 2304-2310 |
| Immunogen | MEK1/MEK2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/MEK2. |
| Modification | p-Ser217 / Ser221 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 MAP2K2 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 ERK activator kinase 1/ERK activator kinase2 MAP kinase kinase 1/MAP kinase kinase 2 MAP2K1/MAP2K1 MAPK/ERK kinase 1/MAPK/ERK kinase 2 MAPKK 1/MAPKK 1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level ofMEK1/MEK2 only when phosphorylated at serine 217/221. |
| Entrez Gene | 5604 / 5605 |
|---|---|
| Protein Accession | NP_002746.1 / NP_109587.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2 (Phospho-Ser217/Ser221).
Application
Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/MEK2 (Phospho-Ser217/Ser221) Antibody#11205
WB
Tan IIA increases the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as detected by immunoblotting.
IHC
Tan IIA increased the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as assessed by immunohistochemical analysis.
WB
(E) Tan IIA increased the expression of p-MEK| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
