Application
Antibody used in IHC on Human Small intestine.Application
Antibody used in WB on Human Jurkat cells at 1 ug/ml.Application
Antibody used in WB on Human brain at 0.2-1 ug/ml.| Product Name | METAP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site.This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Kass, D., (2007) Am. J. Respir. Cell Mol. Biol. 37 (2), 193-201. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human METAP2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| METAP2 antibody can be used for detection of METAP2 by ELISA at 1:312500. METAP2 antibody can be used for detection of METAP2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | METAP2 |
|---|---|
| Alternative Names | METAP2 MAP2 MNPEP p67 p67eIF2 |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in IHC on Human Small intestine.
Application
Antibody used in WB on Human Jurkat cells at 1 ug/ml.
Application
Antibody used in WB on Human brain at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | METAP2 antibody can be used for detection of METAP2 by ELISA at 1:312500. METAP2 antibody can be used for detection of METAP2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store METAP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
