Application
Western blot analysis of METAP2 using rabbit polyclonal METAP2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the METAP2 gene.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | METAP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.1) Xiao, Q., et al. Biochemistry 49(26):5588-5599(2010) |
| Immunogen | This METAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human METAP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | METAP2 |
|---|---|
| Alternative Names | Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175} MAP 2 {ECO:0000255|HAMAP-Rule:MF_03175} MetAP 2 {ECO:0000255|HAMAP-Rule:MF_03175} 341118 {ECO:0000255|HAMAP-Rule:MF_03175} Initiation factor 2-associated 67 kDa glycoprotein {ECO:0000255|HAM |
| Molecular Weight(MW) | 53 kDa |
Application
Western blot analysis of METAP2 using rabbit polyclonal METAP2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the METAP2 gene.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
