Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of METAP2 Antibody in 293 cell line lysates (35ug/lane)| Product Name | Methionine Aminopeptidase 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins. Arf proteins participate in routing of intracellular proteins to and within the Golgi complex. Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.1) Wang, J., et al., Biochemistry 42(17):5035-5042 (2003). |
| Immunogen | This Methionine Aminopeptidase 2 (METAP2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-467 amino acids from the C-terminal region of human Methionine Aminopeptidase 2 (METAP2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | METAP2 |
|---|---|
| Alternative Names | Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175} MAP 2 {ECO:0000255|HAMAP-Rule:MF_03175} MetAP 2 {ECO:0000255|HAMAP-Rule:MF_03175} 341118 {ECO:0000255|HAMAP-Rule:MF_03175} Initiation factor 2-associated 67 kDa glycoprotein {ECO:0000255|HAM |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of METAP2 Antibody in 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
