Application
Immunohistochemistry of MFN2 in rat brain tissue with MFN2 antibody at 5 μg/mL.Application
Immunofluorescence of MFN2 in rat brain tissue with MFN2 antibody at 20 μg/mL.Application
Western blot analysis of MFN2 in human brain tissue lysate with MFN2 antibody at 1 μg/ml.| Product Name | MFN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3,4). Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5).1) Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200. |
| Immunogen | MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2. The immunogen is located within amino acids 250 - 300 of MFN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MFN2 |
|---|---|
| Alternative Names | MFN2 Antibody: HSG MARF CMT2A CPRP1 CMT2A2 KIAA0214 Mitofusin-2 Transmembrane GTPase MFN2 |
| Molecular Weight(MW) | Predicted: 83 kDa Observed: 90 kDa |
| Tissue Specificity | MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1. |
Application
Immunohistochemistry of MFN2 in rat brain tissue with MFN2 antibody at 5 μg/mL.
Application
Immunofluorescence of MFN2 in rat brain tissue with MFN2 antibody at 20 μg/mL.
Application
Western blot analysis of MFN2 in human brain tissue lysate with MFN2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MFN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MFN2 antibody is supplied in PBS containing 0.02% sodium azide. |
