WB
Various lysates were subjected to SDS PAGE followed by western blot with MFN2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.WB
WB result of MFN2 antibody (1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using MFN2 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using MFN2 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Product Name | MFN2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MFN2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC :1:1000-1:4000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MFN2 |
|---|---|
| Gene Synonyms | HSG MSL MARF CMT2A CPRP1 CMT2A2 HMSN6A CMT2A2A CMT2A2B |
| Gene Full Name | mitofusin 2 |
| Gene Summary | This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | 5F3B3 CPRP1 EC:3.6.5.- HSG KIAA0214 |
| Molecular Weight(MW) | 757 aa, 86 kDa |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion outer membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MFN2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB
WB result of MFN2 antibody (1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.
IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using MFN2 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using MFN2 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:5000-1:50000 IHC :1:1000-1:4000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
