WB
Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody at 1/1,000 dilution. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody at 1/1,000 dilution. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Mitofusin 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Mitofusin 2 aa 51-100/757. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MFN2 |
|---|---|
| Gene Synonyms | HSG MSL MARF CMT2A CPRP1 CMT2A2 HMSN6A CMT2A2A CMT2A2B |
| Gene Full Name | mitofusin 2 |
| Gene Summary | This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | CMT2A antibody CMT2A2 antibody CPRP 1 antibody CPRP1 antibody EC 3.6.5.- antibody Fzo antibody HSG antibody hyperplasia suppressor gene antibody Hypertension related protein 1 antibody KIAA0214 antibody MARF antibody MFN 2 antibody Mfn2 antibody MFN2_HUMAN antibody Mitochondrial assembly regulatory factor antibody Mitofusin-2 antibody Mitofusin2 antibody Transmembrane GTPase MFN2 antibody |
| Molecular Weight(MW) | 86 kDa(Observed band size:75 kDa) |
| Cellular Localization | Mitochondrion outer membrane. |
WB
Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody at 1/1,000 dilution. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody at 1/1,000 dilution. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
