Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | MIER1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The association of hMI-ER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMI-ER1 proteins and this may have important implications for hMI-ER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A-sensitive histone deacetylase 1 (HDAC1).1) Paterno, G.D., et al., (1998) Gene 222 (1), 77-82. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MIER1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MIER1 antibody can be used for detection of MIER1 by ELISA at 1:312500. MIER1 antibody can be used for detection of MIER1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MIER1 |
|---|---|
| Alternative Names | MIER1 ER1 MI-ER1 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | MIER1 antibody can be used for detection of MIER1 by ELISA at 1:312500. MIER1 antibody can be used for detection of MIER1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MIER1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
