| Product Name | MIP-1a Antibody [1.2_3E8-2H6-2B6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. |
| Immunogen | The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CCL3 |
|---|---|
| Alternative Names | Macrophage inflammatory protein 1 alpha CCL 3 MIP 1 alpha MIP1a SCYA 3 Heparin binding chemotaxis protein G0S19-1 MIP1A SCYA3 C-C motif chemokine 3 Macrophage inflammatory protein 1-alpha MIP-1-alpha |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MIP-1α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
