Application
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.| Product Name | MIP-3 beta Antibody [G5_4E11.3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Was product number XP-5714-M |
| Immunogen | MIP-3-beta antibody is produced in BALB/c mice using highly pure (>98%) recombinant human MIP-3beta as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with our biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL23 |
|---|---|
| Alternative Names | Macrophage Inflammatory Protein 3 beta SCYA20 Small inducible cytokine A20 LARC Liver and activation regulated chemokine exodus-1 MIP3 MPIF1 SCYA23 C-C motif chemokine 23 CK-beta-8 CKB-8 CKb8 Ckb-8 MIP-3 MPIF-1 Ckb-8-1 hmrp-2a CK-BETA-8 |
Application
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with our biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MIP-3-beta lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
