Application
Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long was detected using purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstratesdetected the MLCK protein (arrow).Application
Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.Application
Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.| Product Name | MLCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Myosin light chain kinase, smooth muscle, MLCK, smMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1 |
| Product description | MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.1) Lazar, V., et al., Genomics 57(2):256-267 (1999). Watterson, D.M., et al., J. Cell. Biochem. 75(3):481-491 (1999). Garcia, J.G., et al., Am. J. Respir. Cell Mol. Biol. 16(5):489-494 (1997). Potier, M.C., et al., Genomics 29(3):562-570 (1995). |
| Immunogen | This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 908-938 amino acids from the N-terminal region of human MLCK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MYLK |
|---|---|
| Alternative Names | Myosin light chain kinase smooth muscle MLCK smMLCK Kinase-related protein KRP Telokin Myosin light chain kinase smooth muscle deglutamylated form MYLK MLCK MLCK1 MYLK1 |
| Molecular Weight(MW) | 211 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rb |
Application
Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long was detected using purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstratesdetected the MLCK protein (arrow).
Application
Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.
Application
Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
