WB
Western blot analysis of MYLK using anti-MYLK antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYLK antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MYLK using anti-MYLK antibody. MYLK was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-MYLK Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of MYLK using anti-MYLK antibody. MYLK was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-MYLK Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to MYLK |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human MYLK recombinant protein (Position: D1441-D1709). Human MYLK shares 98.1% and 97.8% amino acid (aa) sequence identity with mouse and rat MYLK, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYLK |
|---|---|
| Gene Synonyms | KRP AAT7 MLCK MLCK1 MMIHS MYLK1 MMIHS1 MYLK-L smMLCK MLCK108 MLCK210 MSTP083 |
| Gene Full Name | myosin light chain kinase |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] |
| Alternative Names | Kinase related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK1 myosin light chain kinase smMLCK Telokin |
| Molecular Weight(MW) | 211 kDa(Observed band size:135 kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton. |
WB
Western blot analysis of MYLK using anti-MYLK antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYLK antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MYLK using anti-MYLK antibody. MYLK was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-MYLK Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of MYLK using anti-MYLK antibody. MYLK was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-MYLK Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
