WB
FDCA inhibited hypoxia-induced activation of the intracellular Ca2+ signaling pathway. (A) Representative images of [Ca2+]i. (B) Quantitative analysis of Fluo-4 AM fluorescence intensity. (C) Representative Western blot bands. (D) Quantitative analysis of CaMK II/p-CaMK II. (E) Quantitative analysis of p-MLCK/MLCK. Data are expressed as mean ± SD (n=3-5). *P<0.05, **P<0.01 vs normoxia control group; ##P<0.01 vs hypoxia group; &P<0.05, &&P<0.01 vs hypoxia+FDCA group, ns (not significant).| Product Name | Phospho-MLCK (Tyr464) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human MLCK around the phosphorylation site of Tyr464. |
| Modification | p-Tyr464 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:3000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYLK |
|---|---|
| Gene Synonyms | KRP AAT7 MLCK MLCK1 MMIHS MYLK1 MMIHS1 MYLK-L smMLCK MLCK108 MLCK210 MSTP083 |
| Gene Full Name | myosin light chain kinase |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] |
| Alternative Names | deglutamylated form DKFZp686I10125 EC 2.7.11.18 FLJ12216 Kinase related protein Kinase-related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK_HUMAN MYLK1 Myosin light chain kinase Myosin light polypeptide kinase OTTHUMP00000180642 OTTHUMP00000180643 smMLCK smooth muscle Smooth muscle myosin light chain kinase Telokin |
| Molecular Weight(MW) | 211 kDa.; 211kD(Calculated). |
| Cellular Localization | Cytoplasm. Cell projection>Lamellipodium. Cleavage furrow. Cytoplasm>Cytoskeleton>Stress fiber. |
WB
FDCA inhibited hypoxia-induced activation of the intracellular Ca2+ signaling pathway. (A) Representative images of [Ca2+]i. (B) Quantitative analysis of Fluo-4 AM fluorescence intensity. (C) Representative Western blot bands. (D) Quantitative analysis of CaMK II/p-CaMK II. (E) Quantitative analysis of p-MLCK/MLCK. Data are expressed as mean ± SD (n=3-5). *P<0.05, **P<0.01 vs normoxia control group; ##P<0.01 vs hypoxia group; &P<0.05, &&P<0.01 vs hypoxia+FDCA group, ns (not significant).| Application Notes | WB:1:1000-1:3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
