Application
Confocal immunofluorescent analysis of MME Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody .AP7329c was diluted at 1:1000 at each lane.| Product Name | MME Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN |
| Product description | MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.1) Dakka,N., Bellaoui,H. Pediatr Hematol Oncol 26 (4), 216-231 (2009) |
| Immunogen | This MME antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-302 amino acids from the Central region of human MME. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MME |
|---|---|
| Alternative Names | Neprilysin Atriopeptidase Common acute lymphocytic leukemia antigen CALLA Enkephalinase Neutral endopeptidase 2411 NEP Neutral endopeptidase Skin fibroblast elastase SFE CD10 MME EPN |
| Molecular Weight(MW) | 86 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Confocal immunofluorescent analysis of MME Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody .AP7329c was diluted at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
