Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | MNDA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MNDA, PYHIN3 |
| Product description | The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage.MNDA may act as a transcriptional activator/repressor in the myeloid lineage. It plays a role in the granulocyte/monocyte cell-specific response to interferon. MNDA stimulates the DNA binding of the transcriptional repressor protein YY1.The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Briggs, R.C., (2006) Cancer Res. 66 (9), 4645-4651. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MNDA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MNDA antibody can be used for detection of MNDA by ELISA at 1:1562500. MNDA antibody can be used for detection of MNDA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MNDA |
|---|---|
| Alternative Names | MNDA PYHIN3 |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | MNDA antibody can be used for detection of MNDA by ELISA at 1:1562500. MNDA antibody can be used for detection of MNDA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MNDA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
