WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MNDA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MNDA(). Positive lysates(100ug) and (20ug) can be purchased separately from OriGene.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | MNDA Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 37-321 of human MNDA(NP_002423) produced in E.coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:150 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | MNDA |
|---|---|
| Gene Synonyms | PYHIN3 |
| Gene Full Name | myeloid cell nuclear differentiation antigen |
| Gene Summary | The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq, Jul 2008] |
| Alternative Names | PYHIN3 |
| Molecular Weight(MW) | 45.7 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MNDA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MNDA(). Positive lysates(100ug) and (20ug) can be purchased separately from OriGene.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MNDA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | WB:1:2000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
