Application
Immunohistochemical analysis of paraffin - embedded human rectum adenocarcinoma (top) and breast infiltrating carcinoma (bottom) showing nuclear location using Hmof / MYST1 antibody with DAB staining.Application
Western blot analysis using MYST1 monoclonal antibody against truncated MYST1 - His recombinant protein| Product Name | MYST1 Antibody [8C4C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYST1 (MYST histone acetyltransferase 1,MOF) belongs to the MYST family of histone acetyltransferases, which are employed in the cell to bring about transcriptional regulation. The MYST family includes MYST1, is named for the founding members MOZ, yeast YBF2 and SAS2, and TIP60. All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein- protein interactions and targeting transcriptional regulators to chromatin. Although MOF is expressed in both males and females, it associates with the X chromosome only in males. MOF contains a zinc-finger domain that is used to contact the globular part of the nucleosome and histone H4. The carboxy terminal domain of human MOF also has histone acetyltransferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone.1) Sterner, D.E., et al. Microbiol. Mol. Biol 2000 Rev. 64: 435-459. |
| Immunogen | Ni-NTA purified truncated recombinant MYST1-trx expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KAT8 |
|---|---|
| Alternative Names | MOF MYST1 PP7073 Lysine acetyltransferase 8 MYST-1 MOF hMOF ZC2HC8 |
Application
Immunohistochemical analysis of paraffin - embedded human rectum adenocarcinoma (top) and breast infiltrating carcinoma (bottom) showing nuclear location using Hmof / MYST1 antibody with DAB staining.
Application
Western blot analysis using MYST1 monoclonal antibody against truncated MYST1 - His recombinant protein| Application Notes | Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MYST1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
