Application
Immunohistochemistry of NALP12 in human brain tissue with NALP12 antibody at 5 μg/mL.Application
Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 μg/mL.Application
Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 μg/mL.| Product Name | NALP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NALP12 Antibody: NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-κB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.1) Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104. |
| Immunogen | NALP12 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12. The immunogen is located within amino acids 110 - 160 of NALP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NALP12 antibody can be used for detection of NALP12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRP12 |
|---|---|
| Alternative Names | NALP12 Antibody: RNO PAN6 RNO2 FCAS2 NALP12 PYPAF7 CLR19.3 RNO NACHT LRR and PYD domains-containing protein 12 Monarch-1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of NALP12 in human brain tissue with NALP12 antibody at 5 μg/mL.
Application
Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 μg/mL.
Application
Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | NALP12 antibody can be used for detection of NALP12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NALP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NALP12 Antibody is supplied in PBS containing 0.02% sodium azide. |
