Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).Application
NDUFA10 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | NDUFA10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, Complex I-42kD, CI-42kD, NADH-ubiquinone oxidoreductase 42 kDa subunit, NDUFA10 |
| Product description | The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.1) Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009) |
| Immunogen | This NDUFA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human NDUFA10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFA10 |
|---|---|
| Alternative Names | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 mitochondrial Complex I-42kD CI-42kD NADH-ubiquinone oxidoreductase 42 kDa subunit NDUFA10 |
| Molecular Weight(MW) | 41 kDa |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).
Application
NDUFA10 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
