WB
Western blot analysis of NDUFA10 using anti-NDUFA10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFA10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of RT4 cells using anti-NDUFA10 antibody. Overlay histogram showing RT4 cells stained with anti-NDUFA10 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA10 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to NDUFA10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NDUFA10 recombinant protein (Position: H85-R332). Human NDUFA10 shares 91.7% and 90.1% amino acid (aa) sequence identity with mouse and rat NDUFA10, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFA10 |
|---|---|
| Gene Synonyms | CI-42k CI-42KD MC1DN22 |
| Gene Full Name | NADH:ubiquinone oxidoreductase subunit A10 |
| Gene Summary | The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome. [provided by RefSeq, Apr 2016] |
| Alternative Names | NDUFA10 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 mitochondrial Complex I-42kD CI-42kD NADH-ubiquinone oxidoreductase 42 kDa subunit |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of NDUFA10 using anti-NDUFA10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFA10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of NDUFA10 using anti-NDUFA10 antibody. NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-NDUFA10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of RT4 cells using anti-NDUFA10 antibody. Overlay histogram showing RT4 cells stained with anti-NDUFA10 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA10 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
