Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Product Name | NEDD4L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.1) Zhou, R., et al. J. Biol. Chem. 285(40):30523-30530(2010) |
| Immunogen | This NEDD4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-604 amino acids from the Central region of human NEDD4L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEDD4L |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase NEDD4-like 632- NEDD42 Nedd4-2 NEDD4L KIAA0439 NEDL3 |
| Molecular Weight(MW) | 112 kDa |
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
