Application
Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein.| Product Name | Neurofilament NF-L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFL, NF-L, NF68, CMT1F, CMT2E, NFL, 68 kDa neurofilament protein |
| Product description | Neurofilaments are the 10nm or intermediate filament proteins specifically in neurons, and are composed predominantly of three major proteins called NF- NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.1) Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. |
| Immunogen | Preparation of porcine intermediate filaments. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineChickenHumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | NEFL |
|---|---|
| Alternative Names | NFL NF-L NF68 CMT1F CMT2E NFL 68 kDa neurofilament protein |
| Molecular Weight(MW) | 68 |
| Tissue Specificity | Specific for the ~68k Neurofilament L protein. |
Application
Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Neurofilament NF-L antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
