Application
Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibodyat 5 μg/mL.Application
Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibodyat 20 μg/mL.Application
Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.Application
Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.Application
Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.Application
Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.| Product Name | Neurturin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neurturin Antibody: NTN, Neurturin |
| Product description | Neurturin Antibody: Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFRβ, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. NTN binds to and forms a complex with GFRα-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.1) Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470 |
| Immunogen | Neurturin antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Neurturin. The immunogen is located within the last 50 amino acids of Neurturin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Neurturin antibody can be used for detection of neurturin by Western blot. An approximate 14 kDa band of the full length recombinant NTN was detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRTN |
|---|---|
| Alternative Names | Neurturin Antibody: NTN Neurturin |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibodyat 5 μg/mL.
Application
Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibodyat 20 μg/mL.
Application
Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.
Application
Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.
Application
Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.
Application
Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Neurturin antibody can be used for detection of neurturin by Western blot. An approximate 14 kDa band of the full length recombinant NTN was detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Neurturin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Neurturin Antibody is supplied in PBS containing 0.02% sodium azide. |
