NFE2 is required for activity at the locus control region (LCR) upstream of the globin gene complexes.It requires p18 NF-E2 for binding to the NF-E2 motif. NFE2 may play a role in all aspects of hemoglobin production: globin synthesis, heme synthesis, and the procurement of iron.1) McCormack, M.P., (2006) Blood 108 (7), 2248-2256.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFE2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NFE2 antibody can be used for detection of NFE2 by ELISA at 1:312500. NFE2 antibody can be used for detection of NFE2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
NFE2 antibody can be used for detection of NFE2 by ELISA at 1:312500. NFE2 antibody can be used for detection of NFE2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFE2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.